Regulatory

Part:BBa_K2605004:Design

Designed by: Kaitlin Schaaf   Group: iGEM18_Calgary   (2018-10-09)


Chicken beta-globin insulator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 637
    Illegal BamHI site found at 1249
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 152
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part contains a 5' BmtI restriction site and 3' BamHI restriction site so it can be used within our Multiple-Cloning Site flanked by FRT sites Part BBa_K2605002 system.


Source

The most well-characterized insulator is a 1.2kb element from the 5' end of the Gallus gallus (chicken) beta-globin locus and it functions to block enhancer function and to prevent heterochromatin spread (Pikaart, M.J., Recillas-Targa, F., & Felsenfeld, G, 1998). The insulator sequence was found on NCBI (GenBank: U78775.2)

References

Pikaart, M.J., Recillas-Targa, F., Felsenfeld, G. (1998). Loss of transcriptional activity of a transgene is accompanied by DNA methylation and histone deacetylation and is prevented by insulators. Genes and Development, 12, 2852-2862.